The inhibition of Mycoplasma pneumoniae adhesion in a fetuin test system by synthetic analogs and polymeric forms of neuraminic acid
Tokovenko, I.P.; Skripal', I.G.; Malinovskaya, L.P.; Bairamova, N.E.; Mochalova, L.V.; Tuzikov, A.B.; Bovin, N.V.
Mikrobiolohichnyi Zhurnal 56(1): 3-9
1994
ISSN/ISBN: 0201-8462 PMID: 7522086 Document Number: 431977
Eight glycosides and structural analogues of neuraminic acid as well as eight polymeric forms of N-acetyl neuraminic acid have been studied for their inhibitory effect on adhesion of Mycoplasma pneumoniae. Maximum inhibiting effect among low-molecular compounds was manifested by 2 fwdarw 3; sialyllactose which, being used in concentrations 5.0 and 10.0 mu-g/ml, inhibited adhesion of mycoplasmas by 76 and 87%, respectively. These indices for other derivatives in the above mentioned concentrations were as follows (%): 2 fwdarw 6 sialyllactose, 31 and 74%; alpha-Me-glycoside NeuAc, 75 and 85%; alpha-Bn-glycoside-N-trifluoruracetyl NeuAc, 30 and 63%; alpha-Bn-glycoside NeuAc, 32 and 59%; alpha-Bn-glycoside-4-epi-NeuAc, 20 and 27%; beta-Bn-glycoside NeuAc, 2-4%; beta-Me-glycoside NeuAc, 4-5%. The maximum inhibiting effect (50% inhibition at concentration 2.5 mu-mol) among polymeric forms was exerted by the conjugate alpha-benzeneglycoside with polyacrylic acid containing 12 mol % of NeuAc. Conjugates with 8, 16 and 20 mol % of NeuAc possessed a bit less activity. The 50% concentration for them was 5.3, 3.1 and 8.3 mu-mol, respectively. Polymeric forms on the basis of polyacrylamide proved less active.