Antitumor effect of alpha isomer of anordrin in vitro and cell cycle arrest at G1 phase
Weng, S.M.; Xu, Y.P.; Xu, B.
Zhongguo Yao Li Xue Bao 15(1): 47-50
1994
ISSN/ISBN: 0253-9756 PMID: 8010085 Document Number: 431266
Alpha isomer of anordrin (2-alpha, 17-alpha-diethyl-A-norandrostane-2-beta, 17-beta-diol dipropionate, alpha-Ano) inhibited mouse hepatoma (Hep A) and P388 cell growth in vitro. alpha-Ano (20 mu-g cntdot ml-1) inhibited the incorporation of (3H) uridine and (3H) thymidine into RNA and DNA within 3 h, but the inhibition of L-(3H)lysine incorporating into protein was not obvious. alpha-Ano had no effect on the DNA-dependent RNA synthesis with purified nuclei of Hep A cells. It is suggested that the inhibition of RNA and DNA syntheses is the major cause of the cytostatic effect. alpha-Ano blocked the P388 cells at G-1/G-0 phase, and the delay in G-1/G-0 to S phase transition plays an important role in the inhibition of P388 cell growth.