Histopathological changes produced in organs by platelet activating factor
Takiguchi, T.; Luo, X.X.; Ogata, H.; Ishitobi, F.; Iidaka, K.
Masui. Japanese Journal of Anesthesiology 43(8): 1161-1166
1994
ISSN/ISBN: 0021-4892 PMID: 7933496 Document Number: 430618
This experiment was conducted to investigate the effect of platelet activating factor (PAF) on the pathological changes in organs using 10 mice (C-3H/ HeN). Ten mice were divided into two groups of acute and chronic experiment groups. In acute experiment, each mice received iv bolus injection of PAF 2.5 mu-g cntdot kg-1 and in chronic experiment daily ip injection of PAF 7.5 mu-g cntdot kg-1 for 7 days. Histopathology was observed with light microscopy after one hour or after 7 days by hematoxyline and eosin stain. In acute experiment, congestion of the lung, liver, kidney and spleen in all cases, right ventricular dilation in 2 cases and villous necrosis in the small intestine in 4 cases were observed. In chronic experiment, hyaline thrombus with congestion of the lung in 3 cases and congestion of the liver and kidney in all cases were observed. Splenomegaly with increased macrophage was observed, but no necrosis in the small intestine was observed. Marked findings were villous necrosis in the small intestine in acute experiment and hyaline thrombus in the lung as well as splenomegaly in chronic experiment.