Induction of ecto-5'-nucleotidase of rat mesangial cells in culture
Savić V; Vlahović, P.; Stefanović, V.
Srpski Arhiv Za Celokupno Lekarstvo 122(Suppl 1): 24-27
1994
ISSN/ISBN: 0370-8179 PMID: 18173179 Document Number: 424776
It is very well known that macrophages via their secretory products act on many mesangial cell activities. IL-1b (interleukin-1b), tumor necrosis factor alfa (TNF-a) and prostoglandin E2 (PGE2) effect on rat mesangial cell ecto-enzymes: ecto-5'-nucleotidase and ecto-Mg++-ATP-ase were investigated. Ecto-5'-nucleotidase activity was increased in a dose dependent manner after treatment for 24 and 48 hours. Maximum increases reached 4.5 times, 1.7 times and 2.2 times basal values for IL-1b (20 IU/ml), TNF-a (25 ng/ml) and PGE2 (0.1-10 uM) respectively. Ecto-Mg++-ATP-ase activity was unchanged. Cycloheximide (a protein synthesis inhibitor) bloked these stimulatory effects until cyclo-oxigenase inhibitors (indomethacine, ibuprofen and aspirin) diminished activity aproximately 50%. These results indicate that induction of ecto-5'-nucleotidase activity is mainly dependent of protein synthesis and only particulary of increased PGE2 activity.