Monoclonal immunophenotyping of acute lymphoblastic leukemia cells in children using rapid immune alkaline phosphatase
Kolenkova, G.V.; Luk'ianova, A.G.; Lenskaia, R.V.; Kisliak, N.S.; Kenny, M.; Makhartykh, T.Z.; Kondratchik, K.L.; Chernov, V.M.
Gematologiia i Transfuziologiia 39(2): 18-21
1994
ISSN/ISBN: 0234-5730 PMID: 8026682 Document Number: 423848
Monoclonal immunophenotyping of leukemia cells of the bone marrow was carried out by the method of rapid immune alkaline phosphatase (RIAP) in 30 patients with acute lymphoblastic leukemia (ALL) aged 6 months-14 years. The authors used a panel of monoclonal antibodies (MCA) produced by Leu (Belgium) and DAKO (Denmark) directed to antigens of differentiation clusters: Tdt, HLA-DR, CD: 10, 19, 20, 22, 7, 8, 2, 5, 13, 33, 14. The results indicate diversity of compositions of differentiating antigens on leukemia cells of the dominant population and a different degree of leukemic cell pool heterogenicity. RIAP advantages over flow cytometry in immunotyping of ALL cells are shown. Arguments are provided in favor of introduction of broader MCA panel.