Experimental techniques for developing new drugs acting on dementia (1) --Senescence-accelerated mouse: neurochemical approaches and the findings
Nomura, Y.
Nihon Shinkei Seishin Yakurigaku Zasshi 14(3): 139-146
1994
ISSN/ISBN: 1340-2544 PMID: 7941778 Document Number: 422547
The senescence-accelerated mouse (SAM) was established by Takeda et al (1991) as a model of accelerated aging. A P/8 strain of SAM spontaneously shows learning and memory disturbances with increasing age. Several neurochemical markers were examined in the brain of mice of P/8 strain and were compared with those in the R/1 strain as a control. Here, the author describes neurochemical methods and our findings obtained by the methods regarding measurement of: 1) the glutamate content, 2) release of glutamate and acetylcholine (ACh) from brain slices, 3) ligand bindings to neurotransmitter receptors (NMDA, muscarinic ACh), protein kinase C and glial cells and 4) mRNA expression and metabolism of amyloid precursor protein (APP).