The proliferative processes in the skeleton of white rats administered dipal experimentally and after antioxidant therapy with tocopherol
Koveshnikov, V.G.; Pikaliuk, V.S.
Morfologiia 104(3-4): 34-39
1993
ISSN/ISBN: 1026-3543 PMID: 7889158 Document Number: 419037
Specific features of osteogenesis under a toxic action of pesticide Dipal and antioxidation therapy with tocopherol were studied in experiments in inbred albino rats by the method of autoradiography with 3H-thymidine label. The index of labeled nuclei and intensity of the label in chondroblasts and osteoblasts of the epiphyseal cartilage and periosteum of the middle third of the tibia diaphysis were counted in the light autoradiograms. The method of electron autoradiography was used for the identification of DNA-synthesizing osseous and cartilaginous cells and for studying their ultrastructure. The age-related and topographic activity of the proliferative osteogenic processes, their correlation with the degree of the toxic injury of the organism was established. Dipal, as an osteotropic drug, inhibits proliferative processes in the blast osseous and cartilaginous cells, sharply decreasing the index of labeled nuclei and the label intensity. Correction with tocopherol levels the degree of processes of the inhibition of osteogenesis.