Detection of translocation (15;17) using cytogenetic and molecular techniques. Comparative study in 15 patients with acute promyelocytic leukemia
Arranz, E.; Benítez, J.; Santón, A.; Portero, J.A.; Sánchez-Fayos, J.
Sangre 38(5): 355-358
1993
ISSN/ISBN: 0036-4355 PMID: 7908146 Document Number: 418829
To analyze the reproducibility to detect the t (15; 17) with molecular and cytogenetic techniques. Fifteen cases of acute promyelocytic leukaemia were studied by means of cytogenetic techniques (GTG banding) and molecular ones (K/S probe and HindIII, EcoRI and BamHI restriction enzymes). The t (15; 17) was detected with cytogenetic techniques in 60% cases and with molecular ones in 33% cases. Using both (cytogenetic and molecular) techniques the translocation was detected in 73% cases. The use of both techniques may be very useful in order to get a better diagnostic and a closer control of the patient's clinical evolution.