The detection of Pasteurella multocida toxin using a commercially available ELISA
Weber, A.; Wachowitz, R.; Singer, H.; Heil-Franke, G.
Berliner und Munchener Tierarztliche Wochenschrift 106(10): 328-330
1993
ISSN/ISBN: 0005-9366 PMID: 8250823 Document Number: 418054
126 Pasteurella (P.) multocida strains isolated from nasal swabs of pigs were investigated for producing toxin, comparing tissue culture (EBL cells) and DAKO ELISA-PMT-Kit. With help of ELISA 13 toxigenic P. multocida strains were identified, with help of tissue culture 12 strains producing toxin. In 14 of 219 examined nasal swab bacteria cultures of pigs toxin of P. multocida was detected by the commercially available ELISA. Because of an abundant mixed bacteria culture in 7 of these 14 swabs suspected colonies of P. multocida could not be isolated.