Enrichment of estradiol receptor from calf uterus
Dietrich, W.; Wand, H.; Helbing, D.; Görlich, M.
Folia Biologica 39(1): 58-61
1993
ISSN/ISBN: 0015-5500 PMID: 8348987 Document Number: 415862
A three-step procedure to enrich estradiol receptor protein from calf uterus nearly 30,000-fold has been described. Over-all yield is 12%. Control of the single steps has been performed by sodium dodecyl sulfate-electrophoresis, sucrose density gradient centrifugation, gel filtration and determination of receptor quantity. Immunological properties of the preparation obtained have not been controlled.
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