Measurement of the proliferative activity of three different sublines of the Dunning rat prostate tumor R3327
Lohr, F.; Wenz, F.; Flentje, M.; Peschke, P.; Hahn, E.W.
Strahlentherapie und Onkologie Organ der Deutschen Rontgengesellschaft . 169(7): 438-445
1993
ISSN/ISBN: 0179-7158 PMID: 8342118 Document Number: 414984
The development of the BrdUrd assay has greatly facilitated the measurement of proliferation in both human and experimental tumors. The potential doubling time (T-pot) was reported to be the most promising parameter to serve as a predictive assay to select patients for new treatment modalities such as accelerated fractionation and fast neutrons. We compared flow cytometry and immunochemistry applied to three sublines of the Dunning rat prostate tumor with differing differentiation. The anaplastic R3327-AT sublines has a labeling index (LI) of 7 +- 0.5% and 8.8 +- 3.7 measured with flow cytometry and histology respectively, S-phase duration (T-s) of 8 h, a T-pot of 4.7 days and a cell toss factors (PHI) of 15%. Proliferating cells are homogeneously distributed. The moderately well differentiated R3327-HI subline shows a LI of 5% (flow cytometry 5 +- 0.5%, immunohistochemistry 5 +- 2%), T-s of 10.7 h, T-pot of 8.9 days and PHI of 10%. The well differentiated subline R3327-H has two cell populations (DNA-index 1.6) and a labeling index of 3.7 +- 0.5% measured with flow cytometry and 2.2 +- 0.4% with immunochemistry.