Preliminary crystallographic studies on hammerhead ribozymes
Matsumoto, O.; Chen, Y.; Hasegawa, S.; Takenaka, A.; Ohta, T.; Koizumi, M.; Ohtsuka, E.
Nucleic Acids Symposium Series 29: 171
1993
ISSN/ISBN: 0261-3166 PMID: 8247756 Document Number: 414554
Crystallization of hammerhead ribozymes was tried by the hanging drop vapor diffusion method. The crystals grown up to 0.5 x 0.05 x 0.05 mm3 in size gave diffraction patterns with about 5 A resolution using synchrotron radiation. The crystal was determined to be trigonal with space group P3(1) or P3(2) and cell parameters a = b = 49.6 A and c = 53.3 A.