Electron microscopic studies of the effect of x-rays and L-3,4-dihydroxyphenylalanine (L-Dopa) --alone and in combination-- on Harding-Passey melanoma cells in monolayer cell culture
Proske, H.; Schachtschabel, D.O.; Plamper, G.; Paul, N.; Pfab, R.
Strahlentherapie und Onkologie Organ der Deutschen Rontgengesellschaft . 169(8): 500-507
1993
ISSN/ISBN: 0179-7158 PMID: 8356510 Document Number: 414203
Monolayer cultures of Harding-Passey melanoma cells in exponential growth phase were exposed to 8 or 16 Gy by X-ray treatment. The 8 Gy treated cells revealed little ultrastructural changes, while the 16 Gy exposed cells showed increased damage as segregates, swollen mitochondria and vacuoles. Sole treatment with L-3,4-dihydroxyphenylalanine (2 x 10(-4) M L-Dopa) resulted in insignificant electronmicroscopically tangible cell alterations. Combined treatment--starting with 8 Gy irradiation followed by a six-day incubation in the presence of 2 x 10(-4) M L-Dopa--revealed more pronounced cell damage with final cell disintegration; the cytoplasm contained an increased number of vacuoles and segregates, a strongly decreased endoplasmic reticulum as well as swollen mitochondria and less pinocytosis vesicles; the cell surface showed less microvilli. Melanin containing organelles increased after the combination treatment. The growth inhibitory and cell destructive influence of L-Dopa on X-ray pretreated melanogenic melanoma cells was explained with the formation of cytotoxic oxidation products of L-Dopa.