Use of 7-amino-4-methylcoumarin for the spectrophotometric determination of nitrites
Zhou, J.Y.; Dauphin, C.; Hamon, M.
Annales Pharmaceutiques Francaises 51(2): 94-100
1993
ISSN/ISBN: 0003-4509 PMID: 8250489 Document Number: 412439
A new reagent of diazotisation, 7-amino-4-methylcoumarin (coumarin 120) is proposed for the spectrophotometric determination of small amounts of nitrite. To obtain molecules with an high absorptivity, the copulation with different aromatic amines is studied. N, N-dimethylaniline leads to the best results, particularly the reproducibility. Under the optimised conditions, a good linearity (between 10 micrograms/l and 200 micrograms/l) and a good reproducibility (2-3%) according to the amount of detected nitrites are obtained.