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A new 'solid phase' procedure to synthesize immunotoxins (antibody-ribosome inactivating protein conjugates)

Dosio, F.; Brusa, P.; Delprino, L.; Ceruti, M.; Grosa, G.; Cattel, L.; Bolognesi, A.; Barbieri, L.

Farmaco 48(1): 105-115

1993

ISSN/ISBN: 0014-827X
PMID: 8457275
Document Number: 411548
A method to produce immunotoxins (conjugates comprising of a monoclonal antibody and toxin) using ribosome inactivating protein anchored on an affinity gel derivatized with triazinic dye is described. The adsorbed toxins were activated with 2-imino-thiolane and then conjugated to monoclonal antibody activated by SPDP. The "heterogeneous phase" system offered several advantages, reducing the usually required purification steps and opening a way to automatize the conjugation procedure.

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