Cloning and structural analysis of a human thymidylate synthase pseudogene splitted by several Alu sequences
Takeishi, K.; Watanabe, Y.; Isono, K.; Horie, N.
Nucleic Acids Symposium Series 29: 141-142
1993
ISSN/ISBN: 0261-3166 PMID: 8247742 Document Number: 409160
An unidentified genomic DNA fragment of 2.4kb that is weakly hybridizable with thymidylate synthase (TS) cDNA was cloned from a human genomic DNA library. Sequencing of the cloned DNA fragment and comparison of the sequence with that of the known human TS cDNA revealed that the DNA fragment contained a human TS processed pseudogene with unusual features. Based on the rate of nucleotide substitutions for neutral mutations in the 3'-untranslated regions between the gene and the pseudogene, it was estimated that the human TS pseudogene was formed about 16 million years ago.