T cell development: new approaches
Merkenschlager, M.; Fisher, A.G.
Leukemia 7(Suppl): S40-S44
1993
ISSN/ISBN: 0887-6924 PMID: 8361231 Document Number: 407323
The differentiation of T lymphocyte precursors into functioanlly mature progeny procedes in distinct stages. Since these are identified by characteristic constellations of phenotypic markers, the effects of experimental manipulations on T cell development can be readly monitored. In order to complete their development program, thymocytes must interact with stromal elements, which positively and negatively select the functional T cell receptor (TCR) repertoire (1-6). This process normally assures self tolerance and immunocompetence. Disturbances are of practical importance for clinical disorders including immunodeficiencies and autoimmune phenomena (7), raising a particular interest in human T cell development and repertoire formation. Here, we discuss results and possible applications of a culture system for human thymocytes. Further, we describe an in vitro approach addressing the requirements for a crucial step in T cell development; the transition from the immature CD4 CD8 double positive (DP) to the mature CD4 single positive (SP) stage.