Use of a single injection solute-clearance method for determination of glomerular filtration rate and effective renal plasma flow in chickens
Radin, M.J.; Hoepf, T.M.; Swayne, D.E.
Laboratory Animal Science 43(6): 594-596
1993
ISSN/ISBN: 0023-6764 PMID: 8158986 Document Number: 406906
A single-injection clearance method for determining glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) in chickens was compared with the continuous-infusion method. Male and female White Leghorn chickens were anesthetized, and catheters were placed in both brachial veins and over both ureteral openings. A single-bolus injection of 3H-inulin and 14C-para-aminohippuric acid was given at a dose of 1.0 mu-Ci of each solute per kg of body weight and nine blood samples were obtained over 60 min. After the last sample was taken, the birds were given a bolus injection of 1 mu-Ci of 3H-inulin/kg and 1 mu-Ci of 14C-para-aminohippuric acid/kg, followed by a constant infusion of 0.1 mu-Ci of each solute/min at a rate of 0.1 ml cntdot kg-1 cntdot h-1. Following a 45-min stabilization period, three continuous urine collections were performed. A blood sample was obtained at the midpoint of each urine collection. The GFR and ERPF determined by the single-injection method (3.12 +- 0.43 and 22.81 +- 3.48 ml cntdot min-1 cntdot kg-1, respectively) were comparable to values for the continuous-infusion method (2.81 +- 0.35 and 22.88 +- 4.03 ml cntdot min-1 cntdot kg-1, respectively). Both GFR and ERPF determined by the single-injection method correlated with GFR and ERPF determined by the continuous-infusion method. This single-injection method provides a rapid, accurate method for determining GFR and ERPF in chickens and eliminates the need for collection of urine.