Reduction in the susceptibility of Aedes aegypti to Brugia malayi infection after treatment with ethyl methanesulfonate
Rodriguez, P.H.; Lazaro, C.; Castillon, R.
Journal of the American Mosquito Control Association 8(4): 416-418
1992
ISSN/ISBN: 8756-971X PMID: 1474390 Document Number: 404969
The susceptibility to Brugia malayi infections of F-2 and F-4 progenies of Aedes aegypti (Black Eye strain) treated with ethyl methanesulfonate (EMS) was tested. Both 3-day-old males and females were treated with 0.025, 0.050, 0.075 and 0.10% EMS. Control and treated females were then mated with normal or treated males to recover F-1 progeny. F-2 offspring were derived from sibling intercrosses, and 3 lines were established by further intercross matings to generate the F-4. Susceptibility in the 0.025, 0.050, 0.075 and 0.10% EMS F-2s was reduced by 13, 12, 4 and 25%, respectively. The 0.025% and 0.050% EMS F-2 females showed a 29 to 39% decrease in mean L-3 numbers. At 0.075 and 0.10% EMS, mean L-3 numbers decreased by 0.8 and 71%, respectively. The F-4 populations gave overall infections of 65, 56 and 22% for the control, 0.25 and 0.10% EMS lines, respectively. Mean L-3S were reduced by 24 and 77%, respectively, in the 0.025 and 0.10% F-4 EMS-selected populations.