Juxtaglomerular cells cultured on a reconstituted basement membrane
Rayson, B.M.
American Journal of Physiology 262(3 Pt 1): C563-C568
1992
ISSN/ISBN: 0002-9513 PMID: 1312776 Document Number: 403841
Circulating renin levels are regulated by release from juxtaglomerular (JG) cells. Here, for the first time, we describe the primary culture of rat juxtaglomerular cells on a reconstituted basement membrane. In addition, primary cultures were transformed with a temperature-sensitive SV40 large T antigen gene to promote the development of a continuous JG cell line. Both primary cultures and transformed JG cells maintain a highly differentiated state and secrete active renin. These preparations now provide a system in which characterization of the cellular mechanisms of regulation of renin synthesis and release is possible.