Aminopeptidase P: purification of a membrane-bound bradykininase from rat lung
Orawski, A.T.; Simmons, W.H.
Agents and Actions. Supplements 38: 414-421
1992
ISSN/ISBN: 0379-0363 PMID: 1361300 Document Number: 403399
Aminopeptidase P that hydrolyzes the Arg1-Pro2-bond of bradykinin was solubilized from rat lung microsomes using phosphatidylinositol-specific phospholipase C. The enzyme was purified 420-fold by chromatography on decylagarose (two steps), omega-aminodecyl-agarose and DEAE-Sephacel. A single stained band was observed following native gradient (4-15%) polyacrylamide gel electrophoresis. Dipeptidylaminopeptidase IV-like activity was also present in the final preparation and co-migrated with aminopeptidase P in the above gel system.