Immunodetection of tubulin carboxypeptidase activity on nitrocellulose membrane after gel electrophoresis and blotting
Smania, A.M.; Argaraña, C.E.; Weizetfel, J.C.; Barra, H.S.
Biochemistry International 28(5): 921-928
1992
ISSN/ISBN: 0158-5231 PMID: 1288502 Document Number: 401498
It was found that the detyrosination of tryosinated tubulin by tubulin carboxypeptidase can occur when both the enzyme and the substrate are adsorbed on nitrocellulose. This, and the use of a specific antibody that recognizes detyrosinated tubulin allowed us to localize tubulin carboxypeptidase on a nitrocellulose membrane after agarose gel electrophoresis and blotting. The method was also extended to detect pancreatic carboxypeptidase A.