Isolation and characterization of cDNA clones for mouse Ly-9
Sandrin, M.S.; Gumley, T.P.; Henning, M.M.; Vaughan, H.A.; Gonez, L.J.; Trapani, J.A.; McKenzie, I.F.
Journal of Immunology 149(5): 1636-1641
1992
ISSN/ISBN: 0022-1767 PMID: 1506686 Document Number: 401404
We describe the production and characterization of a mouse mAb, S-450-33.2, recognizing the Ly-9.2 specificity. This mAb was used to purify Ly-9 molecules from lymphoid cell lines, and the aminoterminal amino acids were determined. The mAb was also used in a eukaryotic expression system, to isolate cDNA clones encoding Ly-9. Analysis of RNA showed that Ly-9 expression is lymphocyte specific, as determined by the presence of a single hybridizing 2.4-kb species found only in lumphoid cells. Genomic DNA analysis indicated that Ly-9 is encoded by a single-copy gene of 10 to 15 kb. The predicted polypeptide belongs to the Ig superfamily of cell surface molecules with four extracellular Ig like domains, i.e., a non-disulfide-bonded V domain, a truncated C2 domain with two disulfide bounds, a second non-disulfide-bonded V domain, and a truncated C2 domain with two disulfide bonds (V-C2-V-C2). The sequence data also support the view that Ly-9 belongs to the subgroup of the Ig superfamily that includes Bcm-1, CD2, and LFA-3.