The dependence of DNA repair induced by genetically hazardous exposures on the ionic strength of the medium containing the cells
Spitkovskiĭ, D.M.; Ermakov, A.V.; Gorin, A.I.; Pospekhova, N.I.; Prokhorov, A.I.
Tsitologiia 34(7): 76-85
1992
ISSN/ISBN: 0041-3771 PMID: 1475855 Document Number: 400790
The dependence of UV-induced unscheduled DNA synthesis (UDS) in non-stimulated lymphocytes of human peripheral blood on the ionic strength (mu) of the culture medium has been shown. With the level of mu lower or higher than physiological (mu ph) the UDS significantly decreases. The effect of modification of mu due to changes in ionic strength is absent in the lymphocytes of patients with the classic form of xeroderma pigmentosum. This phenomenon may become useful for development of a new test revealing cells with genetically or physiologically changed system of UV-induced DNA repair. Mechanisms of investigated phenomenon, particularly their dependence on the chromatin structure, as well as the influence of ionic strength on binding the repair enzymes with DNA are discussed.