Enzyme techniques in blood group serology: the effect of ionic strength
Campbell, E.J.; Scott, M.L.
Medical Laboratory Sciences 48(1): 52-58
1991
ISSN/ISBN: 0308-3616 PMID: 2062184 Document Number: 372475
Lowering the ionic strength of the solution in which red blood cells were suspended for blood grouping was shown to increase sensitivity and the incidence of 'false' positive results in various papain techniques. A reduction in ionic strength of the solution in which cells were pre-modified increased the titration scores for 'enzyme-only' anti-c, anti-e, anti-E and anti-D. Suspension of papain pre-modified cells in lower strength solutions for antigen/antibody reactions had the same effect.