PDGF AA homodimers are potent chemoattractants for fibroblasts and neutrophils, and for monocytes activated by lymphocytes or cytokines
Shure, D.; Senior, R.M.; Griffin, G.L.; Deuel, T.F.
Biochemical and Biophysical Research Communications 186(3): 1510-1514
1992
ISSN/ISBN: 0006-291X PMID: 1510677 Document Number: 399938
The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells. The mitogenic properties of PDGF AA are well established; however, the chemotactic potential of PDGF AA remains controversial. We now show that PDGF AA is a strong cheomattractant for human monocytes, granulocytes, and fetal bovine ligament fibroblasts. However, highly purified (< 98%) monocytes requrie the addition of lymphocytes or IL-1 for chemotactic responsiveness to PDGF AA but not for full chemotactic activity with formyl-methionyl-leucyl-phenylalanine (fMLP) or C5a. These results indicate that PDGF AA is a potent chemoattractant. These results also indicate that monocytes require activation either by lymphocytes or exogenous cytokines in order to respond chemotactility to PDGF AA but not to fMLP or C5a and suggest roles of the lymphocyte and cytokine in the chemotactic response of the monocyte to PDGF AA in vivo.