Elimination of matrix effects in electrothermal atomic absorption spectrophotometric determinations of bismuth in serum and urine
Dean, S.; Tscherwonyi, P.J.; Riley, W.J.
Clinical Chemistry 38(1): 119-122
1992
ISSN/ISBN: 0009-9147 PMID: 1733583 Document Number: 399683
A sensitive and precise electrothermal atomic absorption spectrophotometric method for determing bismuth concentration is described. Protein precipitation and the use of a palladium modifier reduce the problems of foaming and permit the use of a higher ashing temperature. The detection limit of the assay is 0.9 nmol/L. Total CVs (intraand and interassay) for serum ranged from 3.5% to 15.1 % and for urine from 4.8% to 14.5% at concentrations of 60.0 and 6.0 nmol/L, respectively. Analytical recoveries of bismuth added to serum and urine were 102% and 103% over the same range. The method is robust and reproducible and can be accurately calibrated with aqueous standards.