A comparison of 3 methods of lipoprotein (a) isolation from human plasma
Afanas'eva, O.I.; Adamova, I.I.; Benevodenskaia, G.F.; Pokrovskiĭ, S.N.
Biulleten' Eksperimental'noi Biologii i Meditsiny 113(3): 268-270
1992
ISSN/ISBN: 0365-9615 PMID: 1421223 Document Number: 397274
Three methods for purification of lipoprotein (a) [Lp(a)] from human plasma were compared. Method I: two-stage ultracentrifugation with subsequent gel-filtration of Lp(a) containing fractions (1.063-1.090 g/ml) on Sepharose CL-4B. Method II: ultracentrifugation followed by affinity chromatography of plasma fraction (1.063 g/ml) on anti-apoB sorbent. Method III: affinity chromatography of the whole plasma on anti-apo(a) sorbent. The Lp(a) yield of these methods is 35, 54 and 41%, respectively. The method III is preferable of these three because it permitted high purification of a large amount of Lp(a) by single-step chromatography.