Immunoblot analysis of antibody responses to Shigella dysenteriae type 1 infection
Sinha, A.K.; Chakraborti, S.
Indian Journal of Medical Research 95: 112-114
1992
ISSN/ISBN: 0019-5340 PMID: 1380491 Document Number: 395283
Immunoblot analysis was used to identify the antigenic components of the outer membrane protein (OMP) extract from Sh. dysenteriae type 1 that may be relevant in protection against infection. The OMPs were extracted by ultrasonic disruption followed by Sarkosyl extraction. The macromolecular bands were separated by sodum dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), electroeluted to a nitrocellulose matrix and complexed with peroxidase conjugated antibodies in human convalescent sera. IgG specific reaction was found to a major antigenic component at 57 kilodaltons. In addition, weakly reactive three antigen determinants of > 57 kD and four 57 kD were found. Control sera from a rabbit immunized against OMP also exhibited a similar pattern of antigenic reactivity. Some of the OMPs with high antigenicity may be important in immunoprophylaxis.