Participation of mobile element hobo in transposition events in the long-term instability system of Drosophila melanogaster
Buff, E.M.; Simonova, O.B.; Petruk, S.F.; Gerasimova, T.I.
Genetika 28(12): 73-79
1992
ISSN/ISBN: 0016-6758 PMID: 1338197 Document Number: 394752
The lines with an active hobo elements as well as those without any hobo fragments were hybridized with the y2sc1waG line. This resulted in the appearance of a number of mutations at the white, miniature, and some other loci. The authors analysed, in which way the hobo transposable elements take part in mutagenesis in these crosses. Most of the white mutants obtained were analysed and transpositions of hobo and Stalker elements were demonstrated. Both independent and simultaneous transpositions were found. It was shown by means of the Southern blot analysis that additional hobo or Stalker insertion into or close to the parental unknown waG insertion resulted in mutant white phenotype's shift toward both extreme and partial reversion. Possible participation in mutagenesis of other mobile elements is also under debate.