Effects of androgen on phosphorylation of chromosomal proteins in mouse submandibular gland
Sato, N.; Yoshida, M.; Ota, M.
Biochemistry International 26(5): 843-852
1992
ISSN/ISBN: 0158-5231 PMID: 1610385 Document Number: 394316
The effect of androgen on intranuclear distribution of androgen receptor binding sites was studied. The level of the androgen receptor complex in dinucleosomes increased 1 h after androgen treatment and those in the mono- and trinucleosomes increased 3 h after androgen treatment. Investigation by 32P incorporation into the nuclei and active chromatin showed that androgen resulted in an increase in the rate of phosphorylation at 3 h and reduced to the control level at 6 h. These data suggest that association of androgen receptor with the acceptor sites and activities of protein kinase in active chromatin are simultaneously stimulated for activating the transcription by androgen.