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A combined effect of cortisol and concanavalin a on calcium ion contents in thymic lymphocytes

Sergeev, P.V.; Dukhanin, A.S.; Bulaev, N.N.

Biulleten' Eksperimental'noi Biologii i Meditsiny 113(6): 612-614

1992

ISSN/ISBN: 0365-9615
PMID: 1446030
Document Number: 392623
The influence of hydrocortisone (1-10 microM) on mitogen-induced Ca level was studied in cytoplasma thymocytes of rat. The investigation was conducted with the use of fluorescence technique (FURA-2AM). A specific Ca-blocking effect of the hormone was observed in the thymocytes of intact animals. Hydrocortisone did not change Ca-response of mitogen-stimulated hormone-resistant thymic lymphocytes. A conclusion was made about applicability of this method to assess the response of the cells to glucocorticoids in vitro.

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