Phenotype frequencies of the class Ii (DR, DQ) DNA alleles by the patterns of sequence-specific primer mixtures (SSPM) in four different populations and the probable haplotypes between DRB1 allele and DQB1 allele
Park, M.S.; Tonai, R.
Clinical Transplants 1992: 475-500
1992
ISSN/ISBN: 0890-9016 PMID: 1306720 Document Number: 392440
From this study, it is clear that: 1. Most alleles of DRB1, DRB3, DRB4, DRB5, and DQB1 can be identified by 19 combinations of primers. 2. Among a total of 81 alleles, 54 of 64 were contained in the DRB1, B3, B4 and B5 loci, and all 17 DQB1-locus alleles were identified by this method. From this extensive population study and past serological experience, it is clear that: 3. SSPM, which uses only primers, not probes, is another tool that can be utilized to detect polymorphisms or microvariations in DNA sequence within the same locus.