Light-optical and ultrastructural immunohistochemistry of antigen H-4, a common marker of epithelial cells
Petrov, S.V.; Raĭkhlin, N.T.; Nizamov, R.S.
Biulleten' Eksperimental'noi Biologii i Meditsiny 113(5): 534-536
1992
ISSN/ISBN: 0365-9615 PMID: 1384781 Document Number: 391159
Epitope localization reacting with mice monoclonal antibodies (Mabs) H 4 was investigated using the specimen of epithelium of skin, human uterine cervix as well as in the culture of epithelium cells of guinea-pig duct deferent. Mice monoclonal antibodies against antigen H 4 obtained by the hybridoma method after immunization of mice with rat colon epithelium cytoskeletal fractions were used. Mabs H 4 were shown to react with antigen of intermediate filaments of all studied normal epithelial, cancer cells as well as culture epithelial cells. Mabs H 4 are supposed to be used as a unique immunohistochemical marker of epithelium cells under normal and malignant growth conditions.