Effects of base damages on DNA replication--mechanism of preferential purine nucleotide insertion opposite abasic site in template DNA
Ide, H.; Murayama, H.; Murakami, A.; Morii, T.; Makino, K.
Nucleic Acids Symposium Series 27: 167-168
1992
ISSN/ISBN: 0261-3166 PMID: 1289805 Document Number: 390765
DNA polymerase preferentially inserts purine nucleotides opposite non-instructive lesions such as abasic sites during DNA replication. In order to elucidate the mechanism of the preferential insertion, a DNA template containing a model abasic site and primers containing 4 different nucleotides (A,G,C,T) at primer terminus were synthesized. The stability of the primer terminus nucleotide placed opposite the abasic site was evaluated on the basis of its sensitivity to 3'-5' exonuclease associated with DNA polymerase.