Improvement of the biodegradation of some cellulosic wastes by acid pretreatment
Youssef, K.A.; Ghareib, M.; Nour el Dein, M.M.
Acta Microbiologica Polonica 40(3-4): 187-195
1991
ISSN/ISBN: 0137-1320 PMID: 1726620 Document Number: 387660
Acid pretreatment of cellulosic wastes improved their susceptibility of Fusarium accuminatum enzymes. The effectiveness of acid pretreatment was demonstrated with an increase in both fungal growth and enzyme activities. A growth yield of 0.15 g/100 ml was achieved on medium containing 5% acid pretreated pods of bean for 60 minutes. Avicelase (C-1), carboxymethylcellulase (C-x) and B-glucosidase (C-2) reached their maximal biosynthesis on acid pretreated wheat bran, sugar-cane bagasse and sawdust-containing media, respectively. Xylanase and pectinase attained their highest accumulation on pretreated pods of bean media. A mixture of free sugars has been released by acid pretreatment. 0.199 g dry mycelium was obtained when the fungus was grown on 100 ml of medium containing hydrolysate of 10% H-2SO-4 pretreated pods of bean for 30 min. No cellulase enzymes could be detected on hydrolysate medium at the time that low contents of both xylanase and pectinase were accumulated.