Cyclosporin inhibits phosphate transport and stimulates alkaline phosphatase activity in renal BBMV
Demeule, M.; Béliveau, R.
American Journal of Physiology 260(4 Pt 2): F518-F524
1991
ISSN/ISBN: 0002-9513 PMID: 1672793 Document Number: 385089
The activity of various enzymes and transport systems was studied in renal brush-border membrane vesicles (BBMV) isolated from rats injected daily with cyclosporin. Alkaline phosphatase (AP) was strongly stimulated: 55 an 113% increases were obtained in BBMV isolated from rats injected with 10 mg cyclosporin/kg for 5 and 10 days. The affinity of the enzyme remained unaltered, but maximal activity (Vmax) showed a strong increase of 2.4-fold between control and treated animals. In addition to the phosphatase activity, phosphate binding to AP also showed a dose-dependent stimulation by cyclosporin treatment: 44 and 70% increases in animals treated or 5 days with 5 and 10 mg cyclosporin/kg. However, the activity of aminopeptidase M was not affected by these treatments, and polyacrylamide gel electrophoresis of BBMV revealed no alterations in the profile of membrane proteins, suggesting the specificity of cyclosporin interaction with alkaline phosphatase. Na+-dependent amino acid and D-glucose transport systems remained unaffected by cyclosporin treatment. The Na+-independent transport system for lysine and the Na+-H+ antiporter activity were also unaltered. In contrast, the initial rate of phosphate uptake decreased by 28% after administration of cyclosporin (10 mg/kg) for 5 days: the Michaelis constant (Km) and Vmax decreased from 137 to 85 .mu.M and from 1.49 to 1.07 pmol .cntdot. .mu.g-1 .cntdot. 5 s-1, respectively. "In vitro" studies with membranes isolated from untreated rats were also undertaken by preincubating membranes with cyclosporin. Neither alkaline phophatase nor the transport systems were affected under these conditions. The results indicate the cyclosporin has a specific effect on two activities associated with phosphate metabolism but does not interfere on a short-term basis with renal reabsorption or other solutes or with urinary acidification by the Na+-H+ antiporter in proximal tubules.