Studies on the constituents of Scutellaria species. IV. High performance liquid chromatography of glucuronylflavonoids in Scutellaria ikonnikovii juz
Wang, Y.Q.; Matsuzaki, K.; Takahashi, K.; Okuyama, T.; Shibata, S.
Yao Xue Xue Bao 26(5): 358-361
1991
ISSN/ISBN: 0513-4870 PMID: 1957685 Document Number: 382768
Recently several procedures have been reported for the simultaneous separation of flavonoid glycosides by high performance liquid chromatography (HPLC), and reversed phase column is more suitable than normal phase column. Even so, the simultaneous separation of flavonoids with closely related structures is not easy. We investigated a successful procedure for the simultaneous separation of glucuronyl flavonoids with 5-hydroxyl group by using a reversed phase column combined with gradient elution in the constituent studies of Scutellaria ikonnikovii Juz.. We have also studied the relationship between the retention time and the position of hydroxyls and glucuronyls in the chemical structure.