Effect of pH and preparation method on thermal denaturation of thymus DNA in salt-free solutions
Vlasov, A.P.; Andrianov, V.T.
Biofizika 36(1): 31-34
1991
ISSN/ISBN: 0006-3029 PMID: 1854827 Document Number: 382300
Thermal denaturation of calf thymus DNA was studied by scanning microcalorimetry. It has been shown that long dialysis of DNA against water causes a sharp decrease of DNA thermal stability due to biopolymer protonation. The literature data on the properties of DNA salt-free solutions obtained by dialysis are discussed.
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