Clarification of ascites fluid
Baum, G.; Frey, M.; Santa Teresa, J.
Biotechniques 10(4): 456 458 460
1991
ISSN/ISBN: 0736-6205 PMID: 1651092 Document Number: 381414
An essential first step in the purification of monoclonal antibodies is the clarification of the ascites fluid. While not ignored, this step has not received critical attention. Six different procedures were evaluated with respect to effectiveness and impact on several selected chemical components. For large-scale work, we find an on-line filtration process to be most efficient.
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