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Competitive effect of anti-HLA class I monoclonal antibody (01.65) and N-N-staurosporine on prolipherative response of PHA activated T-lymphocytes

Selvatici, R.; Orlando, P.; Rubini, M.; Balboni, A.; Balugani, S.; Gandini, E.

Biochemistry International 23(1): 53-58

1991

ISSN/ISBN: 0158-5231
PMID: 1863277
Document Number: 380956
N-N-Staurosporine (STAR) inhibits in a dose dependent manner 3H-thymidine (3H-TdR) incorporation in phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMC) treated with anti-HLA class I monoclonal antibody (MAb) 01.65 and its effect results competitive with MAb 01.65. Cytosolic and particulate protein kinase C (PKC) have been studied. Only when PKC particulate activity is no more detectable, the effect of STAR on 3H-TdR incorporation is evident.

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