Investigation on the metabolism of dehydrochloromethyltestosterone in man
Liu, C.S.; Zhang, J.; Zhou, T.H.
Yao Xue Xue Bao 26(9): 682-687
1991
ISSN/ISBN: 0513-4870 PMID: 1821088 Document Number: 380351
A GC-MS method for the investigation on the metabolism of dehydrochloromethyltestosterone (DHCMT) in man is described. Steroids in human urine were adsorbed on a macroporous XAD-2 resin, eluted with methanol, hydrolyzed with glucuronidase, extracted and concentrated for derivatization and then analyzed with GC-MS. DHCMT and its 7 metabolites were detected in urine samples 8 to 30 h after oral administration. Their structures and the variation of their concentration in urine were determined and the possible metabolic pathways of DHCMT were proposed on the basis of GC-MS feature of its metabolites. The specific metabolites and characteristic ions for screening DHCMT positive urine were chosen. Furthermore, the influence of the sample pretreatment methods on GC-MS results has been studied.