Purification of serum albumin with dye-ligand adsorption chromatography
Su, Z.G.; Jiang, W.; Feng, P.S.; Gao, K.Y.
Chinese Journal of Biotechnology 7(2): 161-168
1991
ISSN/ISBN: 1042-749X PMID: 1806026 Document Number: 380075
Dye molecules were coupled to agarose gel beads to form blue, red and yellow chromatography media for the separation of serum albumin. The adsorption properties of five dye-ligands were compared and the effects of temperature, pH, protein concentration and salt concentration were investigated. Human serum albumin (HSA) was purified from human placental plasma by a single step of dye-ligand chromatography under suitable operating conditions. The product was electrophoretically pure, and the elution recovery was as high as 95%. This new technique is superior to the conventional salting-out process.