Expression of human tissue plasminogen activator by recombinant cell lines from various animals
Aleshkov, S.B.; Ustav, M.B.; Baev, A.A.
Molekuliarnaia Biologiia 25(5): 1226-1238
1991
ISSN/ISBN: 0026-8984 PMID: 1753954 Document Number: 379761
A number of recombinant cell lines which produce human tissue plasminogen activator (tPA) was obtained using different hosts--mouse, rat, hamster, simian and human cell lines. All types of recombinant lines secreted active r-tPA into conditioned medium. A slight difference between molecular weights of secreted variants of r-tPA was mediated by the different mechanisms of protein modification. Treatment of some recombinant cell lines with different substances resulted in increased levels of r-tPA production.