Determination of cell nuclear DNA content in polyps and cancerous degeneration in the large intestine
Liu, F.X.
Zhonghua Zhong Liu Za Zhi 13(5): 332-334
1991
ISSN/ISBN: 0253-3766 PMID: 1782842 Document Number: 374547
Nuclear DNA content was measured by microspectrophotometry in 40 bipsy specimens from patients with large bowel polyps (10 juvenile polyps and inflammatory polyps, 25 adenoma subdivided into 3 groups with 10 Grade I, 10 Grade II and 5 Grade III, 5 cancerous degeneration), and 5 normal epithelium of large intestine. The mean DNA value increased steadily as follows: normal epithelium of intestine (13.54 .+-. 1.76 Au); juvenile polyps and inflammatory polyps (14.89 .+-. 0.40 Au); adenoma Grade III (21.96 .+-. 0.92 Au); cancerous dengeneration (24.47 .+-. 1.48 Au). The difference in DNA value in these groups was statistically significant (P < 0.05 .apprx. 0.01). These results suggest that measurement of nuclear DNA content may serve as an objective quantitative parameter for diagnosis of large bowel carcinomatous change of polyps and classification of polypoid adenomas.