Evaluation of the binding of benzo-a-pyrene by blood serum lipoproteins using the cellulose disk method
Poliakov, L.M.; Chasovskikh, M.I.; Panin, L.E.
Ukrainskii Biokhimicheskii Zhurnal 63(1): 101-104
1991
ISSN/ISBN: 0201-8470 PMID: 2028511 Document Number: 371106
A simple and convenient method has been developed for evaluation binding capacity of lipophilic ligands with lipoproteins or other transport proteins. For this purpose cellulose discs (Whatman 3 MM) were loaded with the constant quantity of 3H-benzopyrene and increasing amounts of unlabelled benzopyrene in dimethylsulfoxide, dried up and incubated in solution of lipoproteins. Dissociation constants were deduced from the remained disk radioactivity using the Scatchard plot method. The obtained values of the dissociation constants were: HDL--4.1 x 10(-5) M; LDL--1.5 x 10(-6) M; VLDL--8.8 x 10(-6) M.