Evidence for Campbell's mechanism for fine excision of Tn9 type transposons in Escherichia coli K12
Goryshin, I.I.; Tamm, S.E.; Lantsov, V.A.
Molekuliarnaia Biologiia 25(3): 614-623
1991
ISSN/ISBN: 0026-8984 PMID: 1658617 Document Number: 370124
The plasmid-transposon Tn9-322 was constructed by inverted transposition from the pBR322::Tn9 plasmid. The precise excision of the Tn9-322 transposon from the proB gene site can proceed by the Campbell's model. This fact was demonstrated by appearance of the plasmid-transposons after their precise excision. They contain two IS1 elements flanking a short direct repeat of the target DNA. The recombinational mechanism of precise excision of Tn9 type transposons seems not to be alternative but looks as an additional one to a well-known slippage mechanism proved for Tn5 and Tn10.