Analysis of the polypeptide profile in insulin preparations using HPLC
Streda, M.; Stajner, K.; Stredová, M.
Sbornik Lekarsky 93(9-10): 309-313
1991
ISSN/ISBN: 0036-5327 PMID: 1821464 Document Number: 369838
The purity of insulin preparations which is important during clinical administration of this hormone can be assessed in several ways. The authors suggested and tested a method for analysis of the polypeptide composition of insulin preparations which makes quantitative and qualitative evaluation of the insulin and non-insulin character possible, as well as their separation according to the shape and size of biological macromolecules by gel filtration on HPLC equipment. The described procedure makes possible a more detailed analysis of insulin preparations when the method is used in combination with electromigration and immunological estimations.