Structural analysis of the decanucleotide duplex d (GGTAATTACC) 2 using 1H NMR spectroscopy
Embrey, K.J.; Searle, M.S.; Craik, D.J.
Biochemistry International 24(3): 567-576
1991
ISSN/ISBN: 0158-5231 PMID: 1772434 Document Number: 369461
The conformation of the decanucleotide duplex d(GGTAATTACC)2 has been investigated in solution by one- and two-dimensional proton NMR spectroscopy. Intra- and inter-nucleotide two-dimensional nuclear Overhauser enhancement data, recorded at mixing times between 15 and 250 ms, reveal a right-handed B-DNA structure. The data also show that the A-T basepairs of the TAATTA tract are highly propeller twisted and the minor groove is particularly narrow.