Interaction of EcoRIi restriction and modification enzymes with synthetic DNA fragments. Determination of the size of EcoRIi binding site
Vinogradova, M.V.; Gromova, E.S.; Kosykh, V.G.; Bur'ianov, I.I.; Shabarova, Z.A.
Molekuliarnaia Biologiia 24(3): 847-850
1990
ISSN/ISBN: 0026-8984 PMID: 2402242 Document Number: 367118
EcoRII restriction endonuclease binding site has been determined on the basis of comparison of the binding parameters of the enzyme with synthetic DNA-duplexes of concatemer type containing a different number of EcoRII recognition sites. It has been shown that it consists of 21 +/- 1 base pairs.
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